diff --git a/editor/editor_node.cpp b/editor/editor_node.cpp
index b05c0d85f8..ceff9092fc 100644
--- a/editor/editor_node.cpp
+++ b/editor/editor_node.cpp
@@ -1443,6 +1443,20 @@ void EditorNode::_sources_changed(bool p_exist) {
 		if (!singleton->cmdline_mode) {
 			EditorResourcePreview::get_singleton()->start();
 		}
+
+		// Set initial focus for screen reader users.
+		if (get_tree()->is_accessibility_enabled()) {
+			if (SceneTreeDock::get_singleton()->is_visible_in_tree()) {
+				SceneTreeDock::get_singleton()->get_tree_editor()->get_scene_tree()->grab_focus();
+			} else {
+				TabContainer *tab_container = EditorDockManager::get_singleton()->get_dock_tab_container(SceneTreeDock::get_singleton());
+				if (tab_container) {
+					// Another tab is active (e.g., Import) - focus the tab bar so user can switch.
+					tab_container->get_tab_bar()->grab_focus();
+				}
+			}
+		}
+
 		get_tree()->create_timer(1.0f)->connect("timeout", callable_mp(this, &EditorNode::_remove_lock_file));
 	}
 }